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A dicer-independent miRNA biogenesis pathway that requires Ago catalysis.

Authors: S Cheloufi, CO Dos Santos, MMW Chong, GJ Hannon
E-pub date: 3 Jun 2010
Journal name: Nature

Nondenaturing agarose gel electrophoresis of RNA.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Removal of ribosomal subunits (and rRNA) from cytoplasmic extracts before solubilization with SDS and deproteinization.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Ethanol precipitation of RNA and the use of carriers.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Determining the yield and quality of purified RNA.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Preparation of cytoplasmic and nuclear RNA from tissue culture cells.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Purification of RNA by SDS solubilization and phenol extraction.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc

Removal of DNA from RNA.

Authors: DC Rio, M Ares, GJ Hannon, TW Nilsen
E-pub date: 1 Jun 2010
Journal name: Cold Spring Harb Protoc