Determining the yield and quality of purified RNA.

Abstract:

After any RNA purification by any method, it is necessary to ascertain both the amount (yield) and the integrity (quality) of the RNA obtained. This is essential for any subsequent analysis and critical for any comparative analyses. This article discusses several methods for RNA quantitation and provides tips for each.

Authors:

DC Rio, M Ares, GJ Hannon, TW Nilsen

Journal:

Cold Spring Harb Protoc

Citation info:

2010(6):pdb.top82

Publication date:

1st Jun 2010

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