Variations on a theme: Diverse N-acyl homoserine lactone-mediated quorum sensing mechanisms in Gram-negative bacteria

Abstract:: Many Gram-negative bacteria employ a mechanism of cell-cell communication known as quorum sensing (QS). The role of QS is to enable the cells in a culture to coordinate their gene expression profile with changes in the population cell density. The best characterized mechanisms of QS employ N-acylated homoserine lactones (AHLs) as signalling molecules. These AHLs are made by enzymes known as LuxI homologs, and accumulate in the culture supernatant at a rate proportional to the increase in cell density. Once the AHL concentration exceeds a certain threshold value, these ligands bind to intracellular receptors known as LuxR homologs. The latter are transcriptional regulators, whose activity alters upon binding the AHL ligand, thereby eliciting a change in gene transcription. Over the last five years, it has become increasingly obvious that this is a rather simplistic view of aHL-dependent QS, and that in fact, there is considerable diversity in the way in which LuxI-R homologs operate. The aim ofthe current review is to describe these variations on the basic theme, and to show how functional genomics is revolutionizing our understanding of QS-controlled regulons.

Authors:: D Smith, JH Wang, JE Swatton, P Davenport, B Price, H Mikkelsen, H Stickland, K Nishikawa, N Gardiol, DR Spring, M Welch

Themes