Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

Abstract:

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

Authors:

F Kawasaki, P Murat, Z Li, T Santner, S Balasubramanian

Journal:

Chem Commun (Camb)

Citation info:

53(8):1389-1392

Publication date:

24th Jan 2017

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