Single molecule conformational analysis of DNA G-quadruplexes.

Abstract:

Single molecule fluorescence resonance energy transfer (FRET) can be employed to study conformational heterogeneity and real-time dynamics of biological macromolecules. Here we present single molecule studies on human genomic DNA G-quadruplex sequences that occur in the telomeres and in the promoter of a proto-oncogene. The findings are discussed with respect to the proposed biological function(s) of such motifs in living cells.

Authors:

PS Shirude, S Balasubramanian

Journal:

Biochimie

Citation info:

90(8):1197-1206

Publication date:

31st Aug 2008

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