Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies.
- Abstract:
- We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.
- Authors:
- W Xi, CK Schmidt, S Sanchez, DH Gracias, RE Carazo-Salas, SP Jackson, OG Schmidt
- Journal:
- Nano Lett
- Citation info:
- 14(8):4197-4204
- Publication date:
- 13th Aug 2014
- Full text
- DOI