RNA conformation in catalytically active human telomerase.

Abstract:

We have used single-molecule fluorescence microscopy to study the folded state of human telomerase RNA (hTR). Here we show that hTR adopts a new conformation on binding to human telomerase reverse transcriptase (hTERT) and reconstitution of an active ribonucleoprotein complex. Our data are consistent with the formation of an RNA pseudoknot in active human telomerase.

Authors:

JA Yeoman, A Orte, B Ashbridge, D Klenerman, S Balasubramanian

Journal:

J Am Chem Soc

Citation info:

132(9):2852-2853

Publication date:

10th Mar 2010

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