Protocol for the characterization of the pancreatic tumor microenvironment using organoid-derived mouse models and single-nuclei RNA sequencing.
- Abstract:
- Single-nuclei RNA sequencing (snRNA-seq) allows for obtaining gene expression profiles from frozen or hard-to-dissociate tissues at the single-nuclei level. Here, we describe a protocol to obtain snRNA-seq data of pancreatic tumors from orthotopically grafted organoid-derived mouse models. We provide details on the establishment of these mouse models, the isolation of single nuclei from pancreatic tumors, and the analysis of the snRNA-seq datasets. For complete details on the use and execution of this protocol, please refer to Mucciolo et al.1.
- Authors:
- M Jihad, G Mucciolo, W Li, A Anand, J Araos Henríquez, S Pinto Teles, JS Manansala, S Ashworth, EG Lloyd, PSW Cheng, W Luo, A Sawle, A Piskorz, G Biffi
- Journal:
- STAR Protoc
- Citation info:
- 5(3):103203
- Publication date:
- 20th Sep 2024
- Full text
- DOI