Measurement of Plasma Cell-Free Mitochondrial Tumor DNA Improves Detection of Glioblastoma in Patient-Derived Orthotopic Xenograft Models.
- Abstract:
- The factors responsible for the low detection rate of cell-free tumor DNA (ctDNA) in the plasma of patients with glioblastoma (GBM) are currently unknown. In this study, we measured circulating nucleic acids in patient-derived orthotopically implanted xenograft (PDOX) models of GBM (n = 64) and show that tumor size and cell proliferation, but not the integrity of the blood-brain barrier or cell death, affect the release of ctDNA in treatment-naïve GBM PDOX. Analysis of fragment length profiles by shallow genome-wide sequencing (
- Authors:
- R Mair, F Mouliere, CG Smith, D Chandrananda, D Gale, F Marass, DWY Tsui, CE Massie, AJ Wright, C Watts, N Rosenfeld, KM Brindle
- Journal:
- Cancer Res
- Citation info:
- 79(1):220-230
- Publication date:
- 1st Jan 2019
- Full text
- DOI