G-quadruplex DNA bound by a synthetic ligand is highly dynamic.

Abstract:: Using single molecule fluorescence resonance energy transfer, we investigated the interaction between a quadruplex-binding ligand and the human telomeric G-quadruplex. The binding of quinolinecarboxamide macrocycle to telomeric DNA was essentially irreversible and selectively induced and favored one quadruplex conformation. The ligand-quadruplex complex displayed intramolecular dynamics including quadruplex folding and unfolding in the absence of ligand association and dissociation. We report that the G-quadruplex can be stabilized without preventing the intrinsic intramolecular dynamics of telomeric DNA.

Authors:: PV Jena, PS Shirude, B Okumus, K Laxmi-Reddy, F Godde, I Huc, S Balasubramanian, T Ha

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