Genome wide screen of RNAi molecules against SARS-CoV-2 creates a broadly potent prophylaxis.
- Abstract:
- Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen with over 16,000 RNAi triggers against the SARS-CoV-2 genome using a massively parallel assay to identify hyper-potent siRNAs. We selected 10 candidates for in vitro validation and found five siRNAs that exhibited hyper-potent activity with IC50
- Authors:
- O Yogev, O Weissbrod, G Battistoni, D Bressan, A Naamti, I Falciatori, AC Berkyurek, R Rasnic, M Hosmillo, S Ilan, I Grossman, L McCormick, CC Honeycutt, T Johnston, M Gagne, DC Douek, I Goodfellow, GJ Hannon, Y Erlich
- Publication date:
- 12th Apr 2022
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- DOI