From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis.
- Abstract:
- Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen of over 16,000 RNAi triggers against the SARS-CoV-2 genome, using a massively parallel assay to identify hyper-potent siRNAs. We selected Ten candidates for in vitro validation and found five siRNAs that exhibited hyper-potent activity (IC50
- Authors:
- O Yogev, O Weissbrod, G Battistoni, D Bressan, A Naamati, I Falciatori, AC Berkyurek, R Rasnic, R Izuagbe, M Hosmillo, S Ilan, I Grossman, L McCormick, CC Honeycutt, T Johnston, M Gagne, DC Douek, I Goodfellow, GJ Hannon, Y Erlich
- Journal:
- Commun Biol
- Citation info:
- 6(1):277
- Publication date:
- 16th Mar 2023
- Full text
- DOI