Figure 2 from Imaging Glioblastoma Response to Radiotherapy Using ²H Magnetic Resonance Spectroscopy Measurements of Fumarate Metabolism

Abstract:: 2H MR spectroscopy measurements of labeled fumarate, malate, and water concentrations in A11 (A–E), S2 (F–J), and U87 (K–O) tumors. Tumor spectra were acquired before and 48 hours after the last chemoradiotherapy treatment (temozolomide 100 mg/kg, 20 Gy in total, 5 Gy per fraction). Sum of 12 2H spectra recorded over 60 minutes from A11 (B and D), S2 (G and I), and U87 (L and N) tumors. Injection of [2,3-2H2]fumarate (1 g/kg) started 5 minutes after the start of acquisition of the first spectrum. The peaks were fitted individually using prior knowledge. [2,3-2H2]malate/[2,3-2H2]fumarate ratios before and 48 hours after the last chemoradiotherapy treatment of A11 (n = 3; *, P = 0.0439, paired two-tailed t test; E), S2 (n = 3; ***, P = 0.0006, paired two-tailed t test; J), and U87 (n = 3; **, P = 0.0061, paired two-tailed t test; O). Data are presented as mean ± SD.

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