Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation.

Abstract:

Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 degrees C increase in Tm of the complex.

Authors:

I Horsey, Y Krishnan-Ghosh, S Balasubramanian

Journal:

Chem Commun (Camb)

Citation info:

(17):1950-1951

Publication date:

7th Sep 2002

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