Detection of transgene expression using hyperpolarized 13C urea and diffusion-weighted magnetic resonance spectroscopy.
- Abstract:
- Purpose To assess the potential of a gene reporter system, based on a urea transporter (UTB) and hyperpolarized [13C]urea. Methods Mice were implanted subcutaneously with either unmodified control cells or otherwise identical cells expressing UTB. After injection of hyperpolarized [13C]urea, a spin echo sequence was used to measure urea concentration, T1, and diffusion in control and UTB-expressing tissue. Results The apparent diffusion coefficient of hyperpolarized urea was 21% lower in tissue expressing UTB, in comparison with control tissue (P
- Authors:
- PS Patrick, MI Kettunen, S-S Tee, TB Rodrigues, E Serrao, KN Timm, S McGuire, KM Brindle
- Journal:
- Magnetic Resonance in Medicine
- Citation info:
- 73(4):1401-1406
- Publication date:
- 1st Apr 2015
- Full text
- DOI