Data from Imaging Glioblastoma Response to Radiotherapy Using 2H Magnetic Resonance Spectroscopy Measurements of Fumarate Metabolism
- Abstract:
- <div>Abstract<p>Early detection of tumor cell death in glioblastoma following treatment with chemoradiation has the potential to distinguish between true disease progression and pseudoprogression. Tumor cell death can be detected noninvasively <i>in vivo</i> by imaging the production of [2,3-<sup>2</sup>H<sub>2</sub>]malate from [2,3-<sup>2</sup>H<sub>2</sub>]fumarate using <sup>2</sup>H magnetic resonance (MR) spectroscopic imaging. We show here that <sup>2</sup>H MR spectroscopy and spectroscopic imaging measurements of [2,3-<sup>2</sup>H<sub>2</sub>]fumarate metabolism can detect tumor cell death in orthotopically implanted glioblastoma models within 48 hours following the completion of chemoradiation. Following the injection of [2,3-<sup>2</sup>H<sub>2</sub>]fumarate into tumor-bearing mice, production of [2,3-<sup>2</sup>H<sub>2</sub>]malate was measured in a human cell line–derived model and in radiosensitive and radioresistant patient-derived models of glioblastoma that were treated with temozolomide followed by targeted fractionated irradiation. The increase in the [2,3-<sup>2</sup>H<sub>2</sub>]malate/[2,3-<sup>2</sup>H<sub>2</sub>]fumarate signal ratio posttreatment, which correlated with histologic assessment of cell death, was a more sensitive indicator of treatment response than diffusion-weighted and contrast agent–enhanced <sup>1</sup>H MRI measurements, which have been used clinically to detect responses of glioblastoma to chemoradiation. Overall, early detection of glioblastoma cell death using <sup>2</sup>H MRI of malate production from fumarate could help improve the clinical evaluation of response to chemoradiation.</p>Significance:<p><sup>2</sup>H magnetic resonance imaging of labeled fumarate metabolism can detect early evidence of tumor cell death following chemoradiation, meeting a clinical need to reliably detect treatment response in glioblastoma.</p></div>
- Authors:
- F Hesse, AJ Wright, V Somai, F Bulat, F Kreis, KM Brindle
- Publication date:
- 31st Mar 2023
- Full text
- DOI