Complex stiffness gradient substrates for studying mechanotactic cell migration.

Abstract:

Polyacrylamide gels are cast upon a stiff support with controlled topography, resulting in a thin gel layer of variable height. The topographical profiles project a stiffness map onto the gel, resulting in controlled linear and non-linear 2D stiffness gradients. Fibroblasts, which migrate towards stiffer substrates, accumulate in areas with a gel thickness below 15 μm.

Authors:

C-HR Kuo, J Xian, JD Brenton, K Franze, E Sivaniah

Journal:

Adv Mater

Citation info:

24(45):6059-6064

Publication date:

27th Nov 2012

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