Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer.
- Abstract:
- Chromatin immunoprecipitation-sequencing (ChIP-Seq) is a powerful tool which combines the established method of ChIP with next-generation sequencing (NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.
- Authors:
- KA Holmes, GD Brown, JS Carroll
- Journal:
- Methods Mol Biol
- Citation info:
- 1366:79-98
- Publication date:
- 1st Aug 2016
- Full text
- DOI