Chemical biology of genomic DNA: minimizing PCR bias.

Abstract:

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

Authors:

GR McInroy, E-A Raiber, S Balasubramanian

Journal:

Chem Commun (Camb)

Citation info:

50(81):12047-12049

Publication date:

18th Oct 2014

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