Application of confocal Raman micro-spectroscopy for label-free monitoring of oxidative stress in living bronchial cells
- Abstract:
- Oxidative stress in cancer is implicated in tumor progression, being associated with increased therapy resistance and metastasis. Conventional approaches for monitoring oxidative stress in tissue such as high-performance liquid chromatography and immunohistochemistry are bulk measurements and destroy the sample, meaning that longitudinal monitoring of cancer cell heterogeneity remains elusive. Raman spectroscopy has the potential to overcome this challenge, providing a chemically specific, label free readout from single living cells. Here, we applied a standardized protocol for label-free confocal Raman micro-spectroscopy in living cells to monitor oxidative stress in bronchial cells. We used a quartz substrate in a commercial cell chamber contained within a microscope incubator providing culture media for cell maintenance. We studied the effect of a potent reactive oxygen species inducer, tert-butyl hydroperoxide (TBHP), and antioxidant, N-Acetyl-L-cysteine (NAC) on living cells from a human bronchial epithelial cells (HBEC). We found that the Raman bands corresponding to nucleic acids, proteins and lipids were significantly different (p
- Authors:
- JM Surmacki, I Quirós Gonzalez, SE Bohndiek
- Journal:
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE
- Citation info:
- Vol. 10490
- Publication date:
- 1st Jan 2018
- Full text
- DOI