Altered composition of the γδ T cell pool in lymph nodes during ageing enhances tumour growth
- Abstract:
- ABSTRACT How age-associated decline of immune function leads to increased cancer incidence is poorly understood. Here, we have characterized the cellular composition of the γδ T cell pool in peripheral lymph nodes (pLNs) upon ageing. We found that ageing has minimal cell-intrinsic effects on function and global gene expression of γδ T cells, and TCR γδ diversity remained stable. However, ageing altered TCR δ chain usage and clonal structure of γδ T cell subsets. Importantly, IL-17-producing γδ 17 T cells dominated the γδ T cell pool of aged mice - mainly due to the selective expansion of V γ 6 + γδ 17 T cells and augmented γδ 17-polarisation of V γ 4 + T cells. Expansion of the γδ 17 T cell compartment was supported by increased Interleukin-7 expression in the T cell zone of old mice. In a Lewis lung cancer model, pro-tumourigenic V γ 6 + γδ 17 T cells were exclusively activated in the tumour-draining LN and their infiltration into the tumour correlated with increased tumour size in aged mice. Thus, upon ageing, substantial compositional changes of γδ T cell pool in a dysregulated pLN microenvironment promote tumour growth.
- Authors:
- H-C Chen, N Eling, CP Martinez-Jimenez, LM O’Brien, J Marioni, D Odom, MD la Roche
- Publication date:
- 1st Aug 2018
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- DOI