A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells.

Abstract:

A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of n.m.r. to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by n.m.r. are considered.

Authors:

RJ Simpson, KM Brindle, FF Brown, ID Campbell, DL Foxall

Journal:

Biochem J

Citation info:

202(3):573-579

Publication date:

15th Mar 1982

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