A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging.

Abstract:: A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells. © 2005 Biochemical Society.

Authors:: SW Botchway, I Barba, R Jordan, R Harmston, PM Haggie, S-P Williams, AM Fulton, AW Parker, KM Brindle

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