2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.
- Abstract:
- 5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.
- Authors:
- SM Huber, P van Delft, A Tanpure, EA Miska, S Balasubramanian
- Journal:
- J Am Chem Soc
- Citation info:
- 139(5):1766-1769
- Publication date:
- 8th Feb 2017
- Full text
- DOI