(Note CRUK/Charity/RC & UK gov rates apply to University of Cambridge research groups only)

MS analysis of RIME/IP/affinity enriched samples

• On-bead protease digestion
• Solid phase extraction clean-up of peptides
• Mass spectrometry time
• Raw data processing

CRUK and other Charity Grants (e.g., EC, ERC, WT): £205/sample

RC and UK Government: £295/sample

Full Institute Recovery: £328/sample

TMT-RIME/IP/affinity enriched sample analysis

Relative quantification of RIME samples combining up to 18 individual conditions in a single run.

• On-bead protease digestion
• Solid phase extraction clean-up of peptides
• Isobaric labeling of peptides using TMT tags
• High pH fractionation
• Mass spectrometry time
• Raw data processing

CRUK and other Charity Grants (e.g., EC, ERC, WT): £1,954 

RC and UK Government: £2,715

Full Institute Recovery: £2,964

Full proteome analysis

In-depth identification of proteins from a single sample.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion
• Peptides are separated using a high pH C18 reverse phased chromatography into 20-30 fractions (typically).
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT): £1,422/proteome

RC and UK Government: £2,094/proteome

Full Institute Recovery: £2,362/proteome

Full proteome analysis (FFPE sections)

In-depth identification of proteins from a single sample

• Washes and tissue lysis
• Protein estimation
• Protease digestion 
• Peptides are separated/purified using a high pH C18 reverse phased chromatography (12-15 fractions)
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT): £1,092

RC and UK Government: £1,204

Full Institute Recovery: £1,358

Quantitative (TMT-based) Full proteome analysis

Relative quantification of proteomes using isobaric labels combining up to 18 individual conditions in a single run.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion and labelling with isobaric tags
• Peptides are separated using a high pH C18 reverse phased chromatography into 20-30 fractions (typically).
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

Phosphoproteomic analysis

n-depth phosphoproteomic analysis of a single sample.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion
• Peptides are separated using a high pH C18 reverse phased chromatography into 10-15 fractions (typically).
• Phosphopeptides are enriched by metal ion chromatography
• Each phosphopeptide fraction is analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

Quantitative (TMT-based) Phosphoproteomic analysis

elative quantification of phosphoproteomes using isobaric labels combining up to 18 individual conditions in a single run.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion and labelling with TMT tags
• Peptides are separated using a high pH C18 reverse phased chromatography into 10-15 (typically).
• Phosphopeptides are enriched by metal ion chromatography
• Each phosphopeptide fraction is analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

Combined quantitative (TMT-based) full proteome and phosphoproteome

Relative quantification of proteome and corresponding phoshoproteome using isobaric labels combining up to 18 individual conditions in a single run.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion and labelling with TMT tags
• Peptides are separated using a high pH C18 reverse phased chromatography into 20-30 fraction (typically).
• Phosphopeptides are enriched by metal ion chromatography (10-15 fractions for the enrichment)
• All fractions are analysed individually on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT) (UoC): £4,084

RC and UK Government (UoC) : £6,769

Full Institute Recovery: £7,411

Acetylome analysis

Identification of acetylated peptides from a single sample.

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion 
• Peptides are separated/purified using a high pH C18 reverse phased chromatography
• Acetylome enrichment (4-6 fractions for the enrichment)
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT): £1,873

RC and UK Government: £2,321

Full Institute Recovery: £2,623

Quantitative (TMT-based) acetylome analysis

Relative quantification of acetylated peptides using isobaric labels combining up to 18 individual conditions in a single run

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion 
• TMT labelling
• Peptides are separated/purified using a high pH C18 reverse phased chromatography
• Acetylome enrichment
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT): £3,095

RC and UK Government: £3,990

Full Institute Recovery: £4,375

Combined quantitative (TMT-based) proteome and acetylome

Relative quantification of acetylated peptides using isobaric labels combining up to 18 individual conditions in a single run

• Cell/tissue lysate preparation
• Protein estimation
• Protease digestion 
• TMT labelling
• Peptides are separated/purified using a high pH C18 reverse phased chromatography
• Acetylome enrichment
• Fractions are analysed separately on the mass spectrometer
• Processing of the raw data using Proteome Discoverer

CRUK and other Charity Grants (e.g., EC, ERC, WT): £3,611

RC and UK Government: £4,954

Full Institute Recovery: £5,297