Microscopy

Leica Stellaris 8 – MP

Confocal microscope for high resolution and flexible 3D imaging of live and fixed samples.  

• Extended white light laser (440 nm – 790 nm) and 405 nm laser 

• New sensitive Hybrid detectors including one with far-red sensitivity, photon counting mode (3x HyDS, 1x HyDX, 1x HyDR) 

• Lightning deconvolution 

• TauSense qualitative lifetime imaging  

• Two-Photon imaging for collagen and deep tissue fluorescence imaging with 680 to 1080 nm excitation 

• 8000 Hz resonance scanner 

• AOBS (acoustical optical beam splitter)  

• Navigator and Dye Assistant 

• Spectral detection (excitation and emission scans) 

• High resolution and glycerol objectives

• Transmitted light DIC (40x, 63x, 100x) 

• Stage incubator for live cell imaging 

Leica SP8 – STED

Confocal microscope for high resolution and flexible 3D imaging of live and fixed samples with super-resolution capabilities. 

• White light laser 470 nm – 670 nm

• 405 and 442 nm lasers 

• 5 detectors, 2 PMTs and 3 sensitive HyD detectors, gated 

• 8000 Hz resonance scanner 

• AOBS (acoustical optical beam splitter)  

• Deconvolution with Huygens software 

• STED super-resolution imaging with 594. 660, and pulsed 775 nm depletion lasers 

• Spectral detection (excitation and emission scans) 

• Transmitted light DIC 

• Navigator and Dye assistant 

• Incubation for live cell imaging with CO2 control 

• Cooling stage 

Leica SP5

Confocal microscope for high resolution and flexible 3D imaging of fixed samples. 

• Argon laser (458, 476, 488, 496, 514 nm) 

• 543 nm and 633 nm laser 

• 5 detectors (3x PMT, 2x HyD) 

• 8000 Hz resonance scanner 

• AOBS (acoustical optical beam splitter) 

Dragonfly Spinning Disk

Laser-based Spinning Disk Confocal microscope for fast 3D imaging of fixed and live samples.  

• Lasers: 405, 445, 488, 561, 637 and 730 nm

• two EMCCD cameras Andor iXon 888 

• Stage top incubator with CO2 and humidity control. 

Opera Phenix Plus

Laser-based spinning disc confocal system for high content cell screening and profiling, volumetric imaging of organoids, spheroids and live cell timelapse imaging.  

• Five laser lines at 375 nm, 425 nm, 488 nm, 561 nm and 640 nm.   

• Four cameras offering fast acquisition times.   

• Synchrony optics with enhanced separation of multiple fluorescence channels.  

• Full environmental control including temperature, CO2 and humidity for long term imaging..  

• Automated plate handling robotics and incubator for high throughput imaging of plates or slides and long-time interval imaging of organoids and live cells. 

Operetta CLS

LED-based Widefield and Spinning Disc Confocal system for high content cell screening and profiling, volumetric imaging of organoids, spheroids, and live cell timelapse imaging.

• Eight LEDs at 365 nm, 405 nm, 440 nm, 475 nm. 510 nm, 550 nm, 630 nm and 660 nm.

• Ideal for highly multiplexed immunofluorescence imaging up to 6 colours.

• Environmental control including temperature and  CO2  for long term imaging.​

Zeiss Light Sheet Z1

​​​Fast, gentle multiview imaging of large specimens using Light Sheet Microscopy.

• Lasers: 405, 488, 561 and 638 nm

• 2 cameras pco.edge 4.2 for simultaneous imaging.

• incubation chamber with CO2 control for live imaging.

• Cleared tissue with refractive index of 1.45

Zeiss Widefield 1&2

Widefield imaging with transmitted light brightfield, DIC and reflected light fluorescence with LED excitation.  

• Fully motorized Axio-Observer Z.1 

• Definite Focus 

• Incubation chamber with CO2 control. 

System 1: Fluorescent proteins, fast dynamics 

• Camera: Hamamatsu Orca Falsh 4 

• Light source: Lumencore Spectra X 

• Filter cubes: CFP, YFP, multi-band: DAPI, FITC, Rhod, DIC 

System 2: Standard dyes + ​​​​​​​NIR fluorescence 

• Camera: AxioCam 506 

• Light sources: CoolLED pE 4000 and pE-2 

• Filter cubes: DAPI, GFP, DsRed, RFP, Cy5, Cy7, and DIC  

Software

We provide access to the following commercial image analysis software on our high-performance workstations or remote access servers.  

• Harmony (Revvity, formerly PerkinElmer): High-Content imaging analysis software for Opera Phenix and Operetta CLS microscopes 

• Vision 4D (Zeiss, formerly Arivis): Image analysis for 2D, 3D and 4D data sets, ideal for Light Sheet microscopy data 

• Imaris (Oxford Instruments, formerly Bitplane): 3D and 4D data sets 

• Huygens (Scientific Volume Imaging): Deconvolution of 3D data sets