Flow cytometry

Equipment

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  FACS Aria cell sorter

  High-speed cell sorters are equipped with five lasers, a UV, 407 nm, 488 nm, 561 nm and 640 nm. The optical configuration allows researchers to see four UV, six violet, three green, two blue and three red parameters.

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  S6 Symphony cell sorter

  High parameter, high speed, cell sorter contained within a Class II Biological Safety Cabinet, for sorting unscreened live human cells. Equipped with 6 lasers: 355nm, 405 nm, 488 nm, 561 nm, 640 nm and 808nm. Optical configuration allows for 30 fluorescent markers, 8 UV, 8 violet, 6 blue, 5 yellow/green, 4 red and 1 infra-red excitation.

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  CytoFlex SRT cell sorter

  4 laser, 15 colour, easy to use cell sorter, available after training for scientist to use. Up to 4 way sorting into tubes and plates.

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  FACS Melody cell sorter

  A simple cell sorter that scientists can use independently and is available 24/7 for sorting. 8 colours from the violet/blue/yellow green lasers are particularly suited to fluorescent protein sorts.

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  A5 FACS Symphony

  A very powerful 32 parameter analyser flow cytometer. It has 6 lasers : a UV, violet, blue, yellow/green, red and an infra-red. Fully configured with 30 fluorescence detectors this can allow for very high parameter analysis. Equipped with an HTS plate sampler for automated sample throughput.

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  LSR Fortessa

  An analytical bench top flow cytometer. It has 5 lasers: UV, violet, blue, yellow/green and red, with 18 fluorescence parameters, and is also equipped with the HTS plate sampler.

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  MacsQuant VYB

  This is an analytical bench top flow cytometer. It has 3 lasers: violet, yellow/green and blue (VYB) with 8 fluorescence detectors. Ideal for simple analysis particularly fluorescent proteins and equipped with a built in plate sampler.

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  ImageStreamX-Mk2

  Combining of flow cytometry and microscopy, the Image Stream takes a picture of each cell as it passes through a flow cell and gives each individual fluorescent image or a composite image for analysis. In addition to receiving images, all fluorescence is quantitatively analysed. There are violet, blue, green and red lasers that allow up to 12 parameters that can be observed and saved.

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  Vicells

  Two cell analysers capable of measuring cell viability, cell count, and cell size.

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  Helios mass cytometer

  Mass Cytometry (commonly called CyTOF) uses antibodies labelled with stable metal isotopes instead of fluorescent molecules, with at time-of-flight mass spectrometer as the detector. This allows the instrument to measure 40+ markers at the same time, on the same cell leading to deep phenotyping of each cell in the sample. The large number of available metals also means that barcoding and combining samples can reduce sample staining variability.

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  Hyperion Tissue Imager

  The Helios mass cytometer coupled to the Hyperion tissue imager enables the same metal tagged antibodies to be used to stain tissue sections, again allowing for deep phenotyping with spatial resolution.