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Figure 5 from Photoacoustic Tomography Detects Response and Resistance to Bevacizumab in Breast Cancer Mouse Models

Abstract:
<p><i>Ex vivo</i> histopathologic analysis of the tumor vascular microenvironment explains the PAT findings. <b>A,</b> H&E staining. Scale bar, 1.5 mm. Necrosis was significantly higher in the Bev-R group compared with Ctrl (Ctrl, <i>n</i><sub>tumors</sub> = 14; Bev-NR, <i>n</i><sub>tumors</sub> = 13; Bev-R, <i>n</i><sub>tumors</sub> = 4). <b>B,</b> CD31-positive MVD (vessel/μm<sup>2</sup>) was significantly higher for the Bev-R group compared with the Bev-NR group, which itself was lower than Ctrl (Ctrl, <i>n</i><sub>tumors</sub> = 14; Bev-NR, <i>n</i><sub>tumors</sub> = 13; Bev-R, <i>n</i><sub>tumors</sub> = 5). <b>C,</b> ASMA positivity was significantly higher in both the Bev-NR and Bev-R groups compared with Ctrl (Ctrl, <i>n</i><sub>tumors</sub> = 14; Bev-NR, <i>n</i><sub>tumors</sub> = 14; Bev-R, <i>n</i><sub>tumors</sub> = 4). <b>D,</b> Hypoxyprobe, denoting areas of hypoxia-dependent pimonidazole labeling in the tumor tissue, was significantly higher for the Bev-R group compared with the Bev-NR group, which itself was higher than Ctrl (Ctrl, <i>n</i><sub>tumors</sub> = 13; Bev-NR, <i>n</i><sub>tumors</sub> = 14; Bev-R, <i>n</i><sub>tumors</sub> = 5). <b>E,</b> CAIX positivity, which also reflects tissue hypoxia, was significantly increased in the Bev-R group compared with the Bev-NR group (Ctrl, <i>n</i><sub>tumors</sub> = 13; Bev-NR, <i>n</i><sub>tumors</sub> = 14; Bev-R, <i>n</i><sub>tumors</sub> = 5). <b>F,</b> Coverage of CD31-positive vessels by ASMA staining was significantly lower in the Bev-R group compared with Bev-NR and also lower than Ctrl (Ctrl, <i>n</i><sub>tumors</sub> = 14; Bev-NR, <i>n</i><sub>tumors</sub> = 14; Bev-R, <i>n</i><sub>tumors</sub> = 4). <b>G</b> and <b>H,</b> Toluidine blue–positive objects per mm<sup>2</sup>, reflective of mast cell density in the tumor, were significantly higher in Bev-NR compared with Bev-R and Ctrl groups (Ctrl, <i>n</i><sub>tumors</sub> = 12; Bev-NR, <i>n</i><sub>tumors</sub> = 12; Bev-R, <i>n</i><sub>tumors</sub> = 4; <b>G</b>), though no significant differences in F4/80 staining (<b>H</b>), reflecting macrophages, was observed (Ctrl, <i>n</i><sub>tumors</sub> = 14; Bev-NR, <i>n</i><sub>tumors</sub> = 14; Bev-R, <i>n</i><sub>tumors</sub> = 5). Scale bars, 50 µm. <i>P</i> values are displayed from two-sided Student <i>t</i> tests.</p>
Article has an altmetric score of 89
Authors:
I Quiros-Gonzalez, MR Tomaszewski, MA Golinska, E Brown, L Ansel-Bollepalli, L Hacker, D-L Couturier, RM Sainz, SE Bohndiek
Publication date:
24th Nov 2025
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