A pathway toward clinical translation of hyperpolarized [1,4-13C2,2,3-d2]fumarate as an imaging biomarker for early cellular necrosis in vivo.
- Abstract:
- PURPOSE: The detection of hyperpolarized carbon-13 (HP 13C)-fumarate conversion to 13C-malate using 13C-MRSI is a biomarker for early detection of cellular necrosis. Here, we describe the translation of HP 13C-fumarate as a novel human imaging agent, including the evaluation of biocompatibility and scaling up of the hyperpolarization methods for clinical use. METHODS: Preclinical biological validation was undertaken in fumarate hydratase-deficient murine tumor models and controls. Safety and biocompatibility of 13C-fumarate was assessed in healthy rats (N = 18) and in healthy human volunteers (N = 9). The dissolution dynamic nuclear polarization process for human doses of HP 13C-fumarate was optimized in phantoms. Finally, 2D 13C-MRSI following injection of HP 13C-fumarate was performed in an ischemia-reperfusion porcine kidney model (N = 6). RESULTS: Fumarate-to-malate conversion was reduced by 42%-71% in the knockdown murine tumor model compared to wildtype tumors. Twice-daily injection of 13C-fumarate in healthy rats at the maximum evaluated dose (120 mg/kg/day) showed no significant persistent blood or tissue effects. Healthy human volunteers injected at the maximum dose (3.84 mg/kg) and injection rate (5 mL/s) showed no statistically significant changes in vital signs or blood measurements 1 h post-injection. Spectroscopic evidence of fumarate-to-malate conversion was observed in the ischemic porcine kidney (0.96 mg/kg). CONCLUSION: HP 13C-fumarate has shown promise as a novel and safe hyperpolarized agent for monitoring cellular necrosis. This work provides the basis for future imaging of HP 13C-fumarate metabolism in humans.
- Authors:
- JR Birchall, P Wodtke, A Grimmer, ESS Hansen, LB Bertelsen, N Bøgh, M Wylot, MJ Zamora-Morales, O Arponen, I Horvat-Menih, EC Latimer, F Tan, E Pappa, J Graggaber, J Cheriyan, K Holmes, MJ Locke, H Sladen, J Boren, MI Kettunen, A Chhabra, IB Wilkinson, C Laustsen, K Brindle, MA McLean, FA Gallagher
- Journal:
- Magn Reson Med
- Citation info:
- 94(3):1202-1217
- Publication date:
- 1st Sep 2025
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- DOI