We provide access to state-of-the-art DNA and RNA analysis instruments, methods and applications, in particular next-generation sequencing.

Services: Illumina sequencing

The CRUK Genomics Core Facility provides sequencing on the latest Illumina next-generation sequencing technologies. These allow unbiased genome-wide experiments to be performed that enable researchers to see, at base-pair resolution, what the underlying sequence differences are in cancer genomes.

Services: Library Preparation

For CRUK Cambridge Institute researchers the Genomics facility also provides library preparation services; typically for genomes, exomes, transcriptomes and ChIP-seq experiments. We have a standard list of LP on offer, please discuss your LP needs with us prior to any submissions.

We offer a full service for sequencing, and library prep, where we perform the steps from sample-to-data. We also house equipment for self-service use, where you have access to training, equipment, reagents and consumables, but do the work yourself!

Services: Single cell

Single cell library preparation service is available using 10X Genomics technology platform or with plate-based methods using a low volume liquid handling robot (SPT Labtech Mosquito HV)

Services: booking equipment

Other laboratory equipment in the core is available for trained users and requires booking through the PPMS booking system.

  • Real-Time PCR systems for 384-well plates,QuantStudio 6
    • consumables and some reagents provided in the core, sign out using your account in PPMS
  • Agilent 4200 TapeStation and 4150 TapeStation for automated DNA and RNA electrophoresis of up to 96 or 16 samples respectively.
    • reagents can be signed out using your account in PPMS
  • Agilent 2100 Bioanalyzer for automated DNA and RNA electrophoresis and protein analysis
    • reagents can be signed out using your account in PPMS
  • Covaris S220 ultrasonicator for DNA, RNA or chromatin shearing, tissue homogenization or cell lysis
  • Covaris LE220 high-throughput ultrasonicator for DNA, RNA or chromatin shearing, tissue homogenization or cell lysis, for 1 – 96 samples
  • Fluidigm Juno for hi-plex PCR or amplicon library preparation, as well as RNA-seq (GEX) library prep.
  • Fluidigm Biomark HD: high-throughput microfluidic system for single-cell gene expression analysis, digital PCR, and amplicon-based target enrichment
  • Agilent Bravo Liquid Handling robot used by the core staff for library preparation and qPCR setup
  • SPT Labtech Mosquito HV liquid handler for dispensing reagents

If you have any questions please email our Helpdesk or see Contact us.