Authors:
T Messmer, F von Meyenn, A Savino, F Santos, H Mohammed, ATL Lun, JC Marioni, W Reik
Journal name: 
Cell Rep
Citation info: 
26(4):815-824.e4
Abstract: 
Conventional human embryonic stem cells are considered to be primed pluripotent but can be induced to enter a naive state. However, the transcriptional features associated with naive and primed pluripotency are still not fully understood. Here we used single-cell RNA sequencing to characterize the differences between these conditions. We observed that both naive and primed populations were mostly homogeneous with no clear lineage-related structure and identified an intermediate subpopulation of naive cells with primed-like expression. We found that the naive-primed pluripotency axis is preserved across species, although the timing of the transition to a primed state is species specific. We also identified markers for distinguishing human naive and primed pluripotency as well as strong co-regulatory relationships between lineage markers and epigenetic regulators that were exclusive to naive cells. Our data provide valuable insights into the transcriptional landscape of human pluripotency at a cellular and genome-wide resolution.
DOI: 
http://doi.org/10.1016/j.celrep.2018.12.099
E-pub date: 
31 Dec 2018
Users with this publication listed: 
Aaron Lun
John Marioni