Authors:
GJ Hannon, PA Maroney, JA Denker, TW Nilsen
Journal name: 
Cell
Citation info: 
61(7):1247-1255
Abstract: 
In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5' terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mRNA at an authentic trans splice acceptor site. SL addition occurs via a trans splicing reaction that proceeds through Y-branched intermediates. The branchpoint is located at either of two adenosine residues located 18 and 19 nucleotides upstream of the splice acceptor site.
DOI: 
http://doi.org/10.1016/0092-8674(90)90689-c
Research group: 
Hannon Group
E-pub date: 
31 May 1990
Users with this publication listed: 
Greg Hannon