Authors:
NM Bell, A L'Hernault, P Murat, JE Richards, AML Lever, S Balasubramanian
Journal name: 
Biochemistry
Citation info: 
52(51):9269-9274
Abstract: 
RNA-protein interactions are vital throughout the HIV-1 life cycle for the successful production of infectious virus particles. One such essential RNA-protein interaction occurs between the full-length genomic viral RNA and the major structural protein of the virus. The initial interaction is between the Gag polyprotein and the viral RNA packaging signal (psi or Ψ), a highly conserved RNA structural element within the 5'-UTR of the HIV-1 genome, which has gained attention as a potential therapeutic target. Here, we report the application of a target-based assay to identify small molecules, which modulate the interaction between Gag and Ψ. We then demonstrate that one such molecule exhibits potent inhibitory activity in a viral replication assay. The mode of binding of the lead molecules to the RNA target was characterized by ¹H NMR spectroscopy.
DOI: 
http://doi.org/10.1021/bi401270d
Research group: 
Balasubramanian Group
E-pub date: 
23 Dec 2013