Authors:
AA Tanpure, S Balasubramanian
Journal name: 
Chembiochem
Citation info: 
18(22):2236-2241
Abstract: 
The synthesis of 2'-O-methyl-5-hydroxymethylcytidine (hm5 Cm), 5-hydroxymethylcytidine (hm5 C) and 5-formylcytidine (f5 C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm5 Cm, hm5 C, f5 C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).
DOI: 
http://doi.org/10.1002/cbic.201700492
Research group: 
Balasubramanian Group
E-pub date: 
16 Nov 2017