Authors:
RA Williamson, BJ Smith, S Angal, G Murphy, RB Freedman
Journal name: 
Biochim Biophys Acta
Citation info: 
1164(1):8-16
Abstract: 
Tryptic digests of recombinant TIMP-1 have been resolved on reverse-phase HPLC and the major peaks identified by N-terminal sequencing. This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. The peptide map was used to (i), characterize an insoluble 'core' peptide seen on digestion of TIMP-1 in non-reducing conditions; (ii), confirm the structure of delta 127-184TIMP-1, a recently described truncated form of the TIMP-1 molecule; (iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale.
DOI: 
http://doi.org/10.1016/0167-4838(93)90105-z
E-pub date: 
31 May 1993
Users with this publication listed: 
Gillian Murphy