Authors:
C Ernst, N Eling, CP Martinez-Jimenez, JC Marioni, DT Odom
Journal name: 
Nat Commun
Citation info: 
10(1):1251
Abstract: 
Male gametes are generated through a specialised differentiation pathway involving a series of developmental transitions that are poorly characterised at the molecular level. Here, we use droplet-based single-cell RNA-Sequencing to profile spermatogenesis in adult animals and at multiple stages during juvenile development. By exploiting the first wave of spermatogenesis, we both precisely stage germ cell development and enrich for rare somatic cell-types and spermatogonia. To capture the full complexity of spermatogenesis including cells that have low transcriptional activity, we apply a statistical tool that identifies previously uncharacterised populations of leptotene and zygotene spermatocytes. Focusing on post-meiotic events, we characterise the temporal dynamics of X chromosome re-activation and profile the associated chromatin state using CUT&RUN. This identifies a set of genes strongly repressed by H3K9me3 in spermatocytes, which then undergo extensive chromatin remodelling post-meiosis, thus acquiring an active chromatin state and spermatid-specific expression.
DOI: 
http://doi.org/10.1038/s41467-019-09182-1
Research group: 
Odom Group, Marioni Group
E-pub date: 
28 Feb 2019
Users with this publication listed: 
Christina Ernst
Duncan Odom
John Marioni