Authors:
D Alves, H Li, R Codrington, A Orte, X Ren, D Klenerman, S Balasubramanian
Journal name: 
Nat Chem Biol
Citation info: 
4(5):287-289
Abstract: 
Human telomerase is a ribonucleoprotein that is minimally comprised of protein (hTERT) and RNA (hTR) components. We have applied single-molecule fluorescence two-color coincidence detection to characterize complex formation between fluorophore-labeled components in solution. By systematic labeling and in vitro assembly of hTERT, hTR and telomerase's DNA substrate, we have established that catalytically functional human telomerase comprises a stable hTERT:hTR:substrate interaction in a 1:1:1 absolute stoichiometry.
DOI: 
http://doi.org/10.1038/nchembio.82
Research group: 
Balasubramanian Group
E-pub date: 
31 May 2008