MA Osborne, WS Furey, D Klenerman, S Balasubramanian
Journal name: 
Anal Chem
Citation info: 
The formation and analysis of single molecules of fluorescently labeled DNA immobilized on polystyrene microspheres is described. Analysis by confocal fluorescence microscopy revealed single-step photobleaching, characteristic of a single fluorophore. Microspheres provide a means of locating single molecules by bright-field microscopy, prior to single-molecule detection. This allows the interrogation of single molecules without suffering the limitations of premature photobleaching. Statistical analysis of fluorescence intensities for >100 microspheres suggests attachment of DNA to micropsheres to be consistent with Poisson statistics.
Research group: 
Balasubramanian Group
E-pub date: 
31 Jul 2000
Users with this publication listed: 
Shankar Balasubramanian