Authors:
JG Harrison, X Liu, S Balasubramanian
Journal name: 
Nucleic Acids Res
Citation info: 
27(17):e14
Abstract: 
A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is otherwise hybridised to a complementary strand containing a fluorescent probe. Thus fluorescence generated is related to the affinity of the sample. Competitive analysis of a number of peptide-oligonucleotide conjugates gave data that correlated well with the corresponding UV melting data. The assay will be useful for screening of large numbers of potential single-stranded binding molecules.
DOI: 
http://doi.org/10.1093/nar/27.17.e14
Research group: 
Balasubramanian Group
E-pub date: 
31 Aug 1999
Users with this publication listed: 
Shankar Balasubramanian