Authors:
R Stark, M Grzelak, J Hadfield
Journal name: 
Nat Rev Genet
Abstract: 
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs. However, as next-generation sequencing technologies have developed, so too has RNA-seq. Now, RNA-seq methods are available for studying many different aspects of RNA biology, including single-cell gene expression, translation (the translatome) and RNA structure (the structurome). Exciting new applications are being explored, such as spatial transcriptomics (spatialomics). Together with new long-read and direct RNA-seq technologies and better computational tools for data analysis, innovations in RNA-seq are contributing to a fuller understanding of RNA biology, from questions such as when and where transcription occurs to the folding and intermolecular interactions that govern RNA function.
DOI: 
http://doi.org/10.1038/s41576-019-0150-2
Research group: 
Genomics
E-pub date: 
30 Jun 2019
Users with this publication listed: 
Marta Grzelak