Authors:
CK Kwok, G Marsico, AB Sahakyan, VS Chambers, S Balasubramanian
Journal name: 
Nat Methods
Citation info: 
13(10):841-844
Abstract: 
We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.
DOI: 
http://doi.org/10.1038/nmeth.3965
Research group: 
Balasubramanian Group
E-pub date: 
31 Oct 2016