G Biffi, D Tannahill, J McCafferty, S Balasubramanian
Journal name: 
Nat Chem
Citation info: 
Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function.
Research group: 
Balasubramanian Group
E-pub date: 
31 Mar 2013
Users with this publication listed: 
David Tannahill
Shankar Balasubramanian