Authors:
DC Rio, M Ares, GJ Hannon, TW Nilsen
Journal name: 
Cold Spring Harb Protoc
Citation info: 
2010(6):pdb.prot5438
Abstract: 
This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an ionic detergent that dissolves membranes, disrupts protein-nucleic acid interactions, and inactivates ribonucleases. Once solubilized, addition of phenol or phenol:chloroform:isoamyl alcohol (PCA) completely denatures the protein, and it becomes insoluble in aqueous solution. PCA extraction is the method of choice for preparing cytoplasmic RNA from tissue culture cells or in any other situation (e.g., enzyme reactions) where solubilization in SDS is easily achievable.
DOI: 
http://doi.org/10.1101/pdb.prot5438
Research group: 
Hannon Group
E-pub date: 
01 Jun 2010
Users with this publication listed: 
Greg Hannon