Authors:
HH Paul, KM Brindle, ID Campbell, DJ Smith
Journal name: 
FEBS Lett
Citation info: 
163(2):185-188
Abstract: 
Rat liver mitochondria suspended in buffer made with 2H2O catalyse exchange C-3 proton of added D-3-hydroxybutyrate with solvent deuterons. The kinetics of this process can be followed using spin-echo proton NMR. The observed isotope exchange velocity is sensitive to the activity of D-3-hydroxybutyrate dehydrogenase, which is an integral protein of the inner mitochondrial membrane. A method is described which can be used to obtain the specific isotope exchange velocity of the enzyme in the intact mitochondrion.
DOI: 
http://doi.org/10.1016/0014-5793(83)80815-1
Research group: 
Brindle Group
E-pub date: 
31 Oct 1983
Users with this publication listed: 
Kevin Brindle