GK Marinov, J Wang, D Handler, BJ Wold, Z Weng, GJ Hannon, AA Aravin, PD Zamore, J Brennecke, KF Toth
Journal name: 
Dev Cell
Citation info: 
Huang et al. (2013) recently reported that chromatin immunoprecipitation sequencing (ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Drosophila. Their study also reported that loss of Piwi causes widespread rewiring of transcriptional patterns, as evidenced by changes in RNA polymerase II occupancy across the genome. Here we reanalyze their data and report that the underlying deep-sequencing dataset does not support the authors' genome-wide conclusions.
Research group: 
Hannon Group
E-pub date: 
23 Mar 2015
Users with this publication listed: 
Greg Hannon