Authors:
PM Haggie, KM Brindle
Journal name: 
J Biol Chem
Citation info: 
274(7):3941-3945
Abstract: 
The enzymes of the tricarboxylic acid cycle in the mitochondrial matrix are proposed to form a multienzyme complex, in which there is channeling of substrates between enzyme active sites. However no direct evidence has been obtained in vivo for the involvement of these enzymes in such a complex. We have labeled the tricarboxylic acid cycle enzyme, citrate synthase 1, in the yeast Saccharomyces cerevisiae, by biosynthetic incorporation of 5-fluorotryptophan. Comparison of the 19F NMR resonance intensities from the labeled enzyme in the intact cell and in cell-free lysates indicated that the enzyme is motionally restricted in vivo, consistent with its participation in a multienzyme complex.
DOI: 
http://doi.org/10.1074/jbc.274.7.3941
Research group: 
Brindle Group
E-pub date: 
31 Jan 1999
Users with this publication listed: 
Kevin Brindle