Authors:
FM Barriga, E Montagni, M Mana, M Mendez-Lago, X Hernando-Momblona, M Sevillano, A Guillaumet-Adkins, G Rodriguez-Esteban, SJA Buczacki, M Gut, H Heyn, DJ Winton, OH Yilmaz, CS-O Attolini, I Gut, E Batlle
Journal name: 
Cell Stem Cell
Citation info: 
20(6):801-816.e7
Abstract: 
Highly proliferative Lgr5+ stem cells maintain the intestinal epithelium and are thought to be largely homogeneous. Although quiescent intestinal stem cell (ISC) populations have been described, the identity and features of such a population remain controversial. Here we report unanticipated heterogeneity within the Lgr5+ ISC pool. We found that expression of the RNA-binding protein Mex3a labels a slowly cycling subpopulation of Lgr5+ ISCs that contribute to all intestinal lineages with distinct kinetics. Single-cell transcriptome profiling revealed that Lgr5+ cells adopt two discrete states, one of which is defined by a Mex3a expression program and relatively low levels of proliferation genes. During homeostasis, Mex3a+ cells continually shift into the rapidly dividing, self-renewing ISC pool. Chemotherapy and radiation preferentially target rapidly dividing Lgr5+ cells but spare the Mex3a-high/Lgr5+ population, helping to promote regeneration of the intestinal epithelium following toxic insults. Thus, Mex3a defines a reserve-like ISC population within the Lgr5+ compartment.
DOI: 
http://doi.org/10.1016/j.stem.2017.02.007
Research group: 
Winton Group
E-pub date: 
01 Jun 2017