Authors:
C Feig, ME Peter
Journal name: 
Methods Enzymol
Citation info: 
442:83-100
Abstract: 
Major work elucidating the structure and function of the CD95 death inducing signaling complex (DISC) was carried in the late 1990s. Since then the DISC has become a paradigm for multiprotein signaling complexes in the apoptosis literature. We analyzed the earliest events of DISC formation with a set of different techniques and found a surprising new characteristic of the CD95 DISC. Data revealed that CD95 is palmitoylated on cysteine 199. This lipid modification enhances receptor aggregation to the high molecular weight DISC.
DOI: 
http://doi.org/10.1016/S0076-6879(08)01404-3
E-pub date: 
01 Aug 2008