Authors:
S Stairs, AA Neves, H Stöckmann, YA Wainman, H Ireland-Zecchini, KM Brindle, FJ Leeper
Journal name: 
Chembiochem
Citation info: 
14(9):1063-1067
Abstract: 
Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bioorthogonal isonitrile-tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.
DOI: 
http://doi.org/10.1002/cbic.201300130
Research group: 
Brindle Group
E-pub date: 
17 Jun 2013