Authors:
RL Hesketh, J Wang, AJ Wright, DY Lewis, AE Denton, R Grenfell, JL Miller, R Bielik, M Gehrung, M Fala, S Ros, B Xie, D-E Hu, KM Brindle
Journal name: 
Cancer Res
Abstract: 
Metabolic imaging has been widely used to measure the early responses of tumors to treatment. Here we assess the abilities of positron emission tomography (PET) measurement of [18F]FDG uptake and magnetic resonance imaging (MRI) measurement of hyperpolarized [1-13C]pyruvate metabolism to detect early changes in glycolysis following treatment-induced cell death in human colorectal (Colo205) and breast adenocarcinoma (MDA-MB-231) xenografts in mice. A TRAIL agonist that binds to human but not mouse cells induced tumor-selective cell death. Tumor glycolysis was assessed by injecting [1,6-13C2]glucose and measuring 13C-labeled metabolites in tumor extracts. Injection of hyperpolarized [1-13C]pyruvate induced rapid reduction in lactate labeling. This decrease, which correlated with an increase in histological markers of cell death and preceded decrease in tumor volume, reflected reduced flux from glucose to lactate and decreased lactate concentration. However, [18F]FDG uptake and phosphorylation was maintained following treatment, which has been attributed previously to increased [18F]FDG uptake by infiltrating immune cells. Quantification of [18F]FDG uptake in flow-sorted tumor and immune cells from disaggregated tumors identified CD11b+/CD45+ macrophages as the most [18F]FDG-avid cell type present, yet they represented <5% of the cells present in the tumors and could not explain the failure of [18F]FDG-PET to detect treatment response. MRI measurement of hyperpolarized [1-13C]pyruvate metabolism is therefore a more sensitive marker of the early decreases in glycolytic flux that occur following cell death than PET measurements of [18F]FDG uptake.
DOI: 
http://doi.org/10.1158/0008-5472.CAN-19-0182
Research group: 
Brindle Group
E-pub date: 
30 Apr 2019
Users with this publication listed: 
Kevin Brindle
Marcel Gehrung